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KMID : 0861020080230010075
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Korea Journal of Herbology
2008 Volume.23 No. 1 p.75 ~ p.83
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Effects of Extract of Hyperici Japonici Herba on Lung Cells in Asthma-indused Mice by OVA Exposure
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Lee Young-Yong
Lee Young-Cheol
Seo Young-Bae
Seo Bu-Il
Roh Seong-Soo
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Abstract
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Objectives: The present study was carried out to investigate the effect of Hyperici Japonici Herba on
the proliferation and activation of eosinophils which were prepared from lung cells of asthma-induced
mice by ovalbumin(OVA) treatment.
Methods: C57BL/6 mouse was exposed to OVA three times a week for 6 weeks. The mouse lung
tissues were dissected out, chopped and dessiciated with collagenase(1 ㎍/㎖). Eosinophils were activated by
rIL-3/rmIL-5 co-treatments. The lung cells were treated with extract of Hyperici Japonici Herba(EHH), incubated for 48 hr at 37℃, and analyzed by flow cytometer, ELISA, RT-PCR, immunocytochemistry stain.
Results: The cell number ratio of granulocyte, CD3e-/CCR3+, CD3e+/CD69+, CD4+, CD23+/B220+cells
was increased in rmIL-5/rIL-3 treated control group compared to the normal group. Cells numbers in
the experimental animal group treated with EHH was all decreased.
In ELISA analysis, IL-4, IL-5, IL-13 protein levels and histamine release level were greatly increased
in the control group compared to the normal animal group, then significantly decreased in the
experimental group with 100 ㎍/㎖ of EHH treatment.
In RT-PCR analysis, the HT value of IL-4, IL-5, IL-13, CCR3, Eotaxin were increased in the control
group compared to the normal animal group, then decreased in the experimental group with 100 ㎍/㎖ of
EHH treatment. And eosinophil proliferation levels were 18847±1527(cpm) in the control group,
4676±972(cpm) in the positive control group, and 8675±159(cpm), 11352±1005(cpm), 14325±677(cpm) in the experimental group with 100 ㎍/㎖, 10 ㎍/㎖, 1 ㎍/㎖ of EHH treatment.
Conclusions: The present data suggested that Hyperici Japonici Herba may have an effects on the inhibition of parameters associated with asthma responses in eosinpophils, and thus implicate the possibility for the clinical application of EHH.
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KEYWORD
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OVA, Asthma, EHH (extract of Hyperici Japonici Herba), Eosinophil, RT-PCR, Immunocytochemistry
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